Price list for external users

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NGS Facility equipment lab

Our prices are comparable to those to those of other academic research facilities and typically much cheaper than commercial service providers. We also aim to provide extended bioinformatics advice on how to maximise the information that can be gained from your NGS data.

We currently have Illumina NextSeq 2K, NextSeq 500 and MiSeq machines as well as a PacBio Sequel and NT Minion sequences, each of which produces data in a different quantity, read length and time frame. Please contact us to discuss which is more appropriate for your requirements, if you are uncertain. Since the machines will be upgraded according to the needs of the facility, please check out our current machine specifications if you have not used a service for a while.

Prices from August 2021

This table is designed to give an indication of the current price structure, but please contact us for a detailed price list.

NextSeq 2K, NextSeq 500 and Miseq paired end lanes

MachineLane type: Typical configurationPrice eachComments
MiSeq'Nano', 300 cycle kit: 150 bp paired end£836 (plus VAT)~1 million inserts
MiSeq'Micro', 300 cycle kit: 150 bp paired end£931 (plus VAT)~4 million inserts
MiSeq300 cycle kit: 150 bp paired end£1,325 (plus VAT)~14 million inserts
MiSeq500 cycle kit: 250 bp paired end£1,550 (plus VAT)~14 million inserts
MiSeq600 cycle kit: 300 bp paired end£2,060 (plus VAT)~18 million inserts
MiSeq'v3' 75 bp paired end£1,253 (plus VAT)~18 million inserts
NextSeq 500150 cycle kit: 75 bp paired end (Medium output)£1,390 (plus VAT)~130 million inserts
NextSeq 500300 cycle kit: 150 bp paired end (Medium output)£1,854 (plus VAT)~130 million inserts
NextSeq 50075 cycle kit: 75 bp single end£1,660 (plus VAT)~400 million inserts
NextSeq 500150 cycle kit: 75 bp paired end (High output)£2,632 (plus VAT)~400 million inserts
NextSeq 500300 cycle kit: 150 bp paired end (High output)£3,650 (plus VAT)~400 million inserts
NextSeq 2K300 cycle kit (i.e. 150 bp paired end) (P2)£3,472 (plus VAT)~400 million inserts
NextSeq 2K200 cycle kit (i.e. 100 bp paired end) (P2)£2,766 (plus VAT)~400 million inserts
NextSeq 2K100 cycle kit (i.e. 50 bp paired end or 100 bp single end) (P2)£1,752 (plus VAT)~400 million inserts
NextSeq 2K300 cycle kit (i.e. 150 bp paired end) (P3)£6,164 (plus VAT)~1,100 million inserts
NextSeq 2K200 cycle kit (i.e. 100 bp paired end) (P3)£4,450 (plus VAT)~1,100 million inserts
NextSeq 2K100 cycle kit (i.e. 50 bp paired end or 100 bp single end) (P3)£3,082 (plus VAT)~1,100 million inserts
NextSeq 2KCustom index kit £250 (plus VAT)This required for any NextSeq 2K lanes that needs custom index primers
NextSeq 2KCustom primer kit£250 (plus VAT)This required for any NextSeq 2K lanes that needs custom sequencing primers

Note
NextSeq 2K libraries that require custom sequencing or index primers need custom primer kits. The other sequences don't.
* It is possible to run multiple libraries on a single lane.

Unusual NextSeq or MiSeq run configurations

While lanes tend to generate read lengths of 50, 75, 100, 150, 250 or 300 bp in length, it is possible to vary the the length as long as the combined lengths of read 1 and 2 do not exceed the number of cycles in the kit. However, it is not recommended to produce excessively long reads on the NextSeqs or over 300 bp on the MiSeq. Consequently, we can offer a wide range of run types such as 150 bp single end read or 200 bp read paired to a 100 bp read. If you wish to use an unusual run configuration please feel free to email us to discuss your needs.

Long read sequencing

We are currently developing our long read sequencing capabilities and so we are only displaying rough prices for a sequencing lane. We work with Dr Chris M. Watson who as extensive experience of working with the Minion in a diagnostic setting identifying deleterious structural rearrangements. Similarly, we are developing the skills need to generate PacBio Sequel data for de novo assembly of bacterial and insect sized genomes as well as sequencing difficult to sequence PCR amplicons.

Long read data
One PacBio Sequel lane (long movie time)£960
including VAT
One ONT Minion lane
Due to the licensing conditions, we can't offer minion sequencing as a
for profit service, so it's offered as either a collaboration or as training.
£1440
including VAT
Last updated August 2021

* It is possible to run multiple libraries on a single lane, depending on library type.

Library production costs

If you would prefer to have your libraries made for you, the cost per library of the most popular libraries are shown in the table below. We have extensive experience using a range of library preparation methods, and are very happy to discuss your requirements and answers questions and queries (emails here).

Library type Comments Sample number Price per sample including VAT
PCR based amplicon Library Amplicon libraries :
This library is made by including sequencing adaptors sequences in the PCR primers.
-
NEBNext Ultra DNA Library DNA libraries with no fragmentation step:
This library is typically for small PCR amplicons.
-
NEBNext Ultra DNA Library DNA libraries with a fragmentation step:
This library is typically for long PCR amplicons or whole genomes.
-
Truseq PCR free DNA Library PCR free DNA libraries with a fragmentation step:
This library is typically projects where users wish to avoid sequence bias due to PCR amplification and can extract over 1 ug of DNA per sample.
-
TruSeq Stranded mRNA library mRNA (polyA selection):
This library will not capture RNA molecules without a polyA tail
-
TruSeq Stranded Total RNA Library lnc and mRNA (ribosomal depletion):
This library will contain all long RNA molecules (not tRNA, miRNA, rRNA or mitochondrial)
For degraded samples such as FFPE treated samples, we may suggest using either the NEB rRNA depletion kit or TruSeq RNA Exome library prep kits
-
QIAseq miRNA Library miRNA:
This library will only contain miRNA molecules. Depending on the quality and quantity of input RNA we may use the NEBNext Multiplex Small RNA Library Prep Kit as discussing with the user
-
SureSelect XT Human All Exon V6 Exome and custom capture
This method selects for DNA sequences of interest. This price is for Agilent exome (v6), but can be for any pulldown reagent. However, the price will vary from the exome price shown. Typically a custom pulldown will cost about £3,500 for 16 samples.
-
Last updated August 2021

Consideration for Library production

Guarantee

If the run fails due to failure of the machine as judged by Illumina's diagnostic system, we will re-run your samples free of charge.

On the other hand, refunds will not be issued in the event that data fails to meet your expectations, but the sequencing machine didn't experience any problems.

If you supply your own libraries, we will perform a quality control step on each one and discuss the appropriateness of proceeding with sequencing. Similarly, we will discuss the appropriateness of running a library made by us and if you elect to sequence a library we deem of low quality, we will not be responsible for the outcome. Depending on our assessment of the initial sample quality, we may remake a library at no additional cost if the library creation failed part way through the procedure.

In no instance will we responsible for the cost of producing the initial template sample used to create the library.